UCSF MDI
Symposium
STRUCTURE
& DESIGN OF SYNTHETIC GENE CARRIERS
UCSF Laurel Heights
Conterence Center
San Francisco,
California
February 24-26,
1999
 This
intensive three day meeting addressed key questions of the field: "What
is the structure of current synthetic gene tranfer vehicles and how do
they deliver genes in cells and animals?" and "Can the efficiency and duration
of expression of synthetic gene medicines be improved using new chemical,
computational, and molecular biological techniques?" Organized by Professor
Frank
Szoka (UCSF),
Philip Felgner
(Gene Therapy Systems), and Leaf
Huang (University of Pittsburgh), the meeting was sponsored by the
UCSF Cystic Fibrosis Research and Development Program and the UCSF
Molecular Design Institute. Scientists who devise synthetic gene carriers
and experts at the interface of chemistry, functional biology, and drug
delivery, including computational experts, attended. Selected presenters
of posters were asked to present a synopsis of their work at the meeting.
Speakers
February 24,
1999
*Frank
Szoka, UCSF-Molecular Design Institute
* Cyrus
Safinya, UC-Santa Barbara
* Leaf
Huang, University of Pittsburgh
* Terry Stouch,
Bristol-Myers
Squibb
* Peter
Kollman, UCSF-Molecular Design Institute
* Alexander
Kabanov, University of Nebraska Medical Ctr.
* Nadrian
C. Seeman, New York University
* John
Wagner, Merck
Research Laboratories
* Eric
Alton, Imperial College of Science Technology & Medicine
* Evan
Hersh, Arizona Cancer Center
* Frank
McCormick, UCSF-Cancer Center
February
25, 1999
* Philip
L. Leopold,
New York Hospital/Cornell
Medical Center
* Gergely
L. Lukacs, Hospital for Sick Children Research Institute
* Todd
Giorgio, Vanderbilt University
* David Klatzmann,
Universite Pierre et Marie Curie
* Jean
Michel Vos, University of North Carolina Chapel Hill
* Eric Kmeic,
Thomas Jefferson University
* Jeffrey
Nordstrom,
GeneMedicine
* Hermann
Bujard, ZMBH, University of Heidelberg
February
26, 1999
* Ronald
Zuckerman,
Chiron Corporation
* Vladmir
Budker,
University
of Wisconsin
* Jean Paul
Behr, Centre National de la Recherche Scientifique
* Yasufumi
Kaneda, Osaka University School of Medicine
* Winfried
Wels, Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus
* Philip
Felgner,
Gene Therapy Systems
Program
February 24, 1999
Irwin
Kuntz - Welcome to Meeting
Brigette Sternberg-Papahadjopoulos - Chair
Cyrus
Safinya, "Self Assembled Structures of Lipid-DNA Nonviral Gene Delivery
Systems From Synchrotron X-Ray Diffraction"
Leaf
Huang, "Lipid-Protamine-DNA (LPD) Complex: A Lipopolyplex Formulation"
Terry R. Stouch, "Computer Simulations of
Lipid Bilayers: Current Status and Future Expectations"
Ken Dill - Chair
Peter
Kollman, "Molecular Dynamics Simulations of Nucleic Acid Systems"
Alexander
Kabanov, "Self-Assembly and Activity of Polyplexes"
Nadrian
C. Seeman, "DNA Nanotechnology"
Donald McDonald - Chair
John
A. Wagner, "Adeno-Associated Virus-Mediated Cystic Fibrosis Gene Therapy"
Eric
Alton, "Lipoplexes -Clinical Applications in the Treatment of Cystic
Fibrosis"
Leaf Huang - Chair for Selected Poster
Talks
Lee G. Barron, "Lipoplex-Mediated Gene Delivery
to the Lung Occurs Within 60 Minutes of Intravenous Administration"
Kenneth J. Longmuir, "Use of an Anionic Micelle
Environment to Attenuate Electrostatic Interactions Between DNA and a Cationic
Peptide-Lipid Conjugate to Produce Soluble, Non- Aggregated, Condensed
DNA Complexes"
B. Pitard, "Structure-Function Relationships
of Cationic Lipid/Plasmid DNA Complexes for Gene Therapy"
Nejat Duzgunes, "Human Serum Albumin-Lipoplexes:
Enhanced Gene Delivery and Resistance to Serum"
Christian Plank, "Collagen Sponges as Carriers
for Synthetic Gene Vectors"
POSTER SESSION and DINNER
Mary Anne Koda-Kimble - Welcome to UCSF
Bob Debs - Chair
Evan
Hersh, "Lipoplex Therapy for Cancer"
David H. Kirn, "Oncolytic Adenovirus for Cancer"
February 25, 1999
Alan Verkman - Chair
P.L. Leopold, "Negotiating the Cytoplasm:
Adenoviral Mechanisms for Delivering DNA to the Nucleus"
Gergely
L. Lukacs, "Degradation and Mobility of DNA/DNA Fragments in Cytoplasm"
Todd
Giorgio, "Cellular Mechanisms of Lipoplex DNA"
D. Gruenert - Chair
D. Gruenert, "Restitution of Gene Function
by Site-Specific Targeting with Small DNA Fragments"
Jean-Michel
Vos, "Large Artificial Circular Chimeric Episomes as Models for MAC-based
Gene Therapy andTherapeuctic Animal Transgenesis"
Eric Kmiec, "Reconstructing Genomes by Genetic
Repair"
Y.W. Kan - Chair
Hermann
Bujard, "Controlling Genes Via Tetracyclines: Does the System Hold
Promise for Gene Therapy?"
Jeffrey L. Nordstrom, "The Steroid Regulated
Switch"
Philip
Felgner - Chair for Selected Poster Talks
David H. Thompson, "Acid-Labile Displasmenyl-Based
Cationic Lipid: In Vitro and In Vivo Characterization of Transfection"
Ming Ouyang, "Controlled Template Directed
Assembly of Plasmid DNA into Small Stable Particles with High DNA Concentration"
Brian H. Johnston, "RNA Padlocks: A Potent
New Approach to Gene Inhibition"
Klaus Schughart, "Solvoplexes, A New Type
of Non-Viral Vectors for Intra-Pulmonary Gene Delivery"
POSTER SESSION and COCKTAILS
February 26, 1999
Nejat Duzgunes - Chair
Ronald Zuckermann, "Combinatorial Approach
to DNA Delivery"
Vladimir
Budker, "Self-Assembly of DNA-polymer Complexes Using Template Polymerization"
Jean Paul Behr, "Template Oligomerization
of DNA-bound Cations Produces Calibrated Nanometric Particles"
Frank
Szoka - Chair
Yasufumi Kaneda, "Evolution of a Virosome:
Recent Progress"
Winfried Wels, "Modular Fusion Proteins as
Carriers for Targeted Gene Delivery"
Philip
Felgner, "Peptide Nucleic Acid Dependent Gene Chemistry"
Frank Szoka, Concluding Remarks
LUNCH & DEPARTURE
Poster Images
Background Image by Frank
Szoka is freeze fracture micrograph of a cationic lipid complex with DNA.
Modeling
Image by Thomas Cheatham and Carlos Simmerling (Kollman group) is DNA
dodecamer with counter ions and water in a periodic system.
top
Support
The meeting gratefully acknowledge the financial
support of Avanti Polar Lipids,
Biodelivery Sciences, Chiron,
Gene
Therapy Systems, GeneMedicine,
Genetic Therapy, Genzyme, Megabios,
Glaxo
Wellcome, Qiagen, Transgene, the UCSF Molecular Design Institute, UCSF
Cystic Fibrosis Research Development Program, and UC Systemwide Biotechnology
Research and Education Program.
UCSF
MDI Scientific Meetings Series
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